Online and in situ monitoring of environmental pollutants: electrochemical biosensing of cadmium.
نویسندگان
چکیده
Online sensitive monitoring of gene expression is essential for understanding microbial life and microbial communities, especially under stress-inducing conditions, such as the presence of environmental pollutants. We describe here a novel use of promoter-based electrochemical biosensing for online and in situ monitoring of gene expression in response to pollutants. As a model system, we used a cadmium-responsive promoter from Escherichia coil fused to a promoterless lacZ gene, which was monitored using an electrochemical assay of beta-galactosidase activity. This whole-cell biosensor could detect, within minutes, nanomolar concentrations of cadmium in water, sea water and soil samples, and it can be used for continuous online and in situ monitoring.
منابع مشابه
Disposable single-use electrochemical sensor: A novel hollow fiber based tool for environmental monitoring of cadmium
The objective of this study is to design a simple, fast, sensitive and single-use electrode with the simultaneous capability of preconcentration and measuring, for application in a three-electrode voltammetry system to identify and measure the heavy metal cadmium. The design process of this sensor consists of several stages. The polyurethane foam and multi walled carbon nanotubes nanoparticles ...
متن کاملElectrochemical sensors for environmental monitoring: design, development and applications.
The advancement in miniaturization and microfabrication technology has led to the development of sensitive and selective electrochemical devices for field-based and in situ environmental monitoring. Electrochemical sensing devices have a major impact upon the monitoring of priority pollutants by allowing the instrument to be taken to the sample (rather than the traditional way of bringing the s...
متن کاملApplication of Graphene and Graphene Oxide for modification of electrochemical sensors and biosensors: A review
This paper gives a comprehensive review about the most recent progress in graphene and graphene oxide based electrochemical sensors and biosensors. Graphene, emerging as a true 2-dimensional material, has received increasing attention due to its unique physicochemical properties (high surface area, excellent conductivity, high mechanical strength, and ease of functionalization and mass product...
متن کاملHistopathological response of hepatopancreas and gill in Metopograpsus messor crab exposed to cadmium pollutants
In recent years, monitoring the amount of pollutants in aquatic ecosystems by quantity measures in water, sediment and fish tissue has shifted to the quality measures of pollutant effects on aquatic animals and assessment of aquatic animal health and, ultimately, the ecosystem. This research performed on three replicates has examined the histopathological response of hepatopancreas and gill in ...
متن کاملRemoval of heavy metals (lead, cadmium, zinc, nickel and iron) from water by bio-ceramic absorbers of hydroxy-apatite microparticles
Heavy metals are highly poisonous in the environment, even in small quantities, and endanger certain species and all live beings. Current methods of removing heavy metals from aqueous media include chemical sequestration, ion exchange, surface absorption, membrane processes, oxidation and revival procedures which have high investment and exploitation costs. Hence, it is significantly necessary ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Environmental microbiology
دوره 2 3 شماره
صفحات -
تاریخ انتشار 2000